International Standard
ISO 23418:2022
Microbiology of the food chain — Whole genome sequencing for typing and genomic characterization of bacteria — General requirements and guidance
Reference number
ISO 23418:2022
Edición 1
2022-06
International Standard
Vista previa
ISO 23418:2022
75509
No disponible en español
Publicado (Edición 1, 2022)

ISO 23418:2022

ISO 23418:2022
75509
Idioma
Formato
CHF 173
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Resumen

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:

a) handling of bacterial cultures;

b) axenic genomic DNA isolation;

c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;

d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;

e) metadata capture and sequence repository deposition;

f) validation of the end-to-end WGS workflow (fit for purpose for intended application).

This document is applicable to bacteria isolated from:

—    products intended for human consumption;

—    products intended for animal feed;

—    environmental samples from food and feed handling and production areas;

—    samples from the primary production stage.

Informaciones generales

  •  : Publicado
     : 2022-06
    : Norma Internacional publicada [60.60]
  •  : 1
     : 45
  • ISO/TC 34/SC 9
    07.100.30 
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